m barkeri 227  (ATCC)

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Unrooted phylogenetic tree for nifH gene products prepared using the neighbor-joining method as described in Materials and Methods. Sequences in clusters II and III are more highly represented than are those in cluster I, and the two nifH sequences from M. barkeri 227 are in boldface.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques:

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Physical map of a 3.4-kb DNA fragment from M. barkeri 227 containing nifHDGK1 genes. The original clone of Sibold et al. (25) contained nifH1, ORF105, ORF 123, and a small part of nifD1. The PCR oligonucleotide primers (termed oligo1 and oligo2), used for PCR cloning of the nifDGK1 genes, are indicated by arrows. The direction of transcription for the nif genes is shown by a large arrow. H, HindIII; E, EcoRI; P, PstI; EV, EcoRV.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques: PCR Cloning

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Unrooted phylogenetic tree for nifD gene products prepared using the neighbor-joining method. The nifD gene products from M. barkeri 227 are in boldface.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques:

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Unrooted tree prepared using the neighbor-joining method showing phylogeny of vnfG/anfG gene products. The “nifG1” sequence from M. barkeri 227 is in boldface.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques: Sequencing

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Effects of additions of trace metals on methanogenesis by growing cultures of M. barkeri 227. All cultures except the culture designated No addition received a vitamin mixture (1). Except in the case of addition of NH4+, the cultures were grown diazotrophically. Error bars indicate standard deviations.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques:

Journal:

Article Title: Analysis of Genes Encoding an Alternative Nitrogenase in the Archaeon Methanosarcina barkeri 227

doi:

Figure Lengend Snippet: Effects of W on methanogenesis by M. barkeri 227 grown diazotrophically in the presence of Mo or V. Error bars indicate standard deviations.

Article Snippet: M. barkeri 227 (ATCC 43241, DSM 1538, and OCM 35) was obtained from our own culture collection, and DNA was extracted and purified from it as described previously ( 7 ).

Techniques: